FG beads are also used in the following applications:
Identifying the interactions between proteins is very important to understand the function of proteins of unknown function. By analyzing the target protein, recovered using protein-immobilized beads, the function of the immobilized protein can be clarified.
Intelectin1 ameliorates macrophage activation via inhibiting the nuclear factor kappa B pathway
Endocrine Journal 69 (2022) 5 Pages 539-546
Chemical Synthesis of Atomically Tailored SUMO E2 Conjugating Enzymes for the Formation of Covalently Linked SUMO–E2–E3 Ligase Ternary Complexes.
J. Am. Chem. Soc., 141, 37, 14742(2019)
Affinity Chromatographic Materials.
Encyclopedia of Polymeric Nanomaterials, DOI:10.1007 (2014).
Protein fishing using magnetic nano-beads containing calmodulin site-specifically immobilized via an azido-group
J. Biochem., DOI:10.1093 (2013).
Disruption of TBP-2 ameliorates insulin sensitivity and secretion without affecting obesity
Nat. Commun., 1:127 (2010).
The enteropathogenic E. coli effector EspB facilitates microvillus effeacing and anitiphagocytosis by inhibiting myosin function
Cell Host & Microbe, 2, 383 (2007).
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
Nucleic Acids Res., 11, 1475 (1983).
By using an antibody that recognizes antigens on the cell surface, FG beads can easily recover cells in a short time by magnetic recovery and does not require a large device. You can select either the direct method in which the antibody is immobilized on FG beads and then added to the cells, or the indirect method in which the cells are first labeled with the antibody and then the beads are added.
Cell separation
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Age-related dysfunction of p53-regulated phagocytic activity in macrophages
Biochemical and Biophysical Research Communications 529 (2020) 462
With beads with DNA or RNA immobilized, you can do the following:
Another method is to introduce a functional group to the end of DNA or RNA and immobilize it.
Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding RNAs
Epigenetics & Chromatin (2021) 14:46
Itpr1 regulates the formation of anterior eye segment tissues derived from neural crest cells
Development (2021) 148 (16): dev188755
DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells
Chemistry & Biology 22 (2015) 1671–1679
Comparative analysis of type 2 diabetes-associated SNP alleles identifies allele-specific DNA-binding proteins for the KCNQ1 locus
Int J Mol Med. 2015 Jul;36(1):222-30.
Affinity Chromatographic Materials.
Encyclopedia of Polymeric Nanomaterials, DOI:10.1007 (2014).
M. Golan-Mashiach et al. (2012)
Identification of CTCF as a master regulator of the clustered protocadherin genes
Nucl. Acids Res., 40, 3378 (2012).
A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress
Genes Dev., 23, 208 (2009).
Embryonic Lethality, Decreased Erythropoiesis, and Defective Octamer-Dependent Promoter Activation in Oct-1-Deficient Mice
Mol. Cell. Biol., 24, 1022 (2004).
Regulation of Immunoglobulin Promoter Activity by TFII-I Class Transcription Factors
J. Biol. Chem., 279, 5460 (2004).
Total Analysis and Purification of Cellular Proteins Binding to Cisplatin-Damaged DNA Using Submicron Beads
Bioconjugate Chem., 13, 163 (2002).
Selective isolation of DNA or RNA using single-stranded DNA affinity latex particles
J. Colloid Interface Sci., 177, 245 (1996).
Copurification of casein kinase II with transcription factor ATF/E4TF3
Nucleic Acids Res., 24, 876 (1996).
[40] DNA affinity chromatography
Methods in Enzymology, 254, 595 (1995).
Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex
J. Biomater. Sci. Polym. Ed., 5, 293 (1994).
Direct purification of multiple ATF/E4TF3 polypeptides from HeLa cell crude nuclear extracts using DNA affinity latex particles
Anal. Biochem., 206, 109 (1992).
A Common trans-Acting Factor, Ad4-binding Protein, to the Promoters of Steroidogenic P-450s
J. Biol. Chem., 267, 17913 (1992).
Purification of DNA-binding transcription factors by their selective adsorption on the affinity latex particles
Nucleic Acids Res., 17, 6229 (1989).
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
Nucleic Acids Res., 11, 1475 (1983).
Antibodies that recognize virus and exosome surface antigens are immobilized on beads and isolated.
Quick and ultra-sensitive digital assay of influenza virus using sub-picoliter microwells
Analytica Chimica Acta Volume 1213, 22 June 2022, 339926
Y. Kabe et al. (2019) [ExoCounter]
Application of high-performance magnetic nanobeads to biological sensing devices.
Aal. Bioanal. Chem., doi: 10.1007/s00216-018-1548-y (2019).
SR Kristensen et al. (2018) [ExoCounter]
Blu-ray beyond music and movies—novel approach to diagnostics measuring specific extracellular vesicles
Journal of Laboratory and Precision Medicine 84 (2018) 3
Y. Kabe et al. (2018) [ExoCounter]
Development of a Highly Sensitive Device for Counting the Number of Disease-Specific Exosomes in Human Sera
Clinical Chemistry, DOI: 10.1373/clinchem.2018.291963 (2018).
Development of an efficient entire-capsid-coding-region amplification method for direct detection of poliovirus from stool extracts
J. Clin. Microbiol., 53, 73 (2015).
Tsujita et al.(2013) [ExoCounter]
Ultrahigh-Sensitivity Biomarker Sensing System Based on the Combination of Optical Disc Technologies and Nanobead Technologies
Japanese Journal of Applied Physics 52 (2013) 09LB02
Development of poliovirus extraction method from the stool extracts by using magnetic nanoparticles sensitized with soluble poliovirus receptor.
J. Clin. Microbiol. DOI:10.1128 (2013).
The target protein is immobilized on the beads and purified using phages with peptides (or proteins) that interact specifically with the target protein.
Directed evolution of orthogonal RNA–RBP pairs through library-vs-library in vitro selection
Nucleic Acids Research, gkab527 (2021)
Combinatorially Screened Peptide as Targeted Covalent Binder:Alteration of Bait-Conjugated Peptide to Reactive Modifier
Bioconjugate Chem., 29, 1866 (2018)
