Identifying the interactions between proteins is very important to understand the function of proteins of unknown function. By analyzing the target protein, recovered using protein-immobilized beads, the function of the immobilized protein can be clarified.
By using an antibody that recognizes antigens on the cell surface, FG beads can easily recover cells in a short time by magnetic recovery and does not require a large device. You can select either the direct method in which the antibody is immobilized on FG beads and then added to the cells, or the indirect method in which the cells are first labeled with the antibody and then the beads are added.
With beads with DNA or RNA immobilized, you can do the following:
Identification of proteins, such as transcription factors that specifically bind to specific base sequences of DNA and RNA
Identification of complementary DNA or RNA that specifically binds to single-stranded DNA or RNA
Immobilization of double-stranded DNA
Prepare double-stranded DNA having 4 to 10 bases of guanine at the protruding end, which easily bind to epoxy groups, and immobilize it on Plain beads. To avoid the effects of steric hindrance, we recommend a base pair of about 500.
Immobilization of single-stranded DNA / RNA
Since there is a possibility that various parts of DNA and RNA will be immobilized on the beads, if they are single-stranded, they are immobilized on the beads with double strands and then dissociated to form single strands.
Another method is to introduce a functional group to the end of DNA or RNA and immobilize it.