Please tell me how to separate FG beads (magnetic separation and centrifugation).
In order to reduce the background (reduce non-specific adsorption), the following cautions are required in the experiments.
Immobilization
Perform centrifugal separation.
The nano size of FG beads gives them excellent dispersibility. As a result, magnetic separation in an organic solvent may be difficult, and it is necessary to recover the FG beads by centrifugal separation.
During affinity purification (screening)
Perform magnetic separation
When centrifugal separation is performed, heavy proteins and insoluble proteins are also precipitated, raising the level of the background. Because FG beads have high dispersibility, magnetic separation requires time (in some cases 5 minutes or longer). However using magnetic separation avoids the risk of intrusion by these impurities and provides clear results with a low background level.
