FF beads/ FS beads
FG beads containing fluorescent dye.
There are two types, “FF beads” that have a magnetic material that can be separated magnetically, and “FS beads” that do not have a magnetic material.
There are three types of fluorescent dyes: Europium and 2 types of Cyanine compounds.
Comparison of FF beads and FS beads
About fluorescent dyes
In the case of Europium
Europium is characterized by a long-life fluorescent dye life and a very long Stokes shift.
In addition, since the fluorescent dye emits very strong light, it can be expected to be used for high sensitivity of in vitro diagnostic agents.
Europium (Excitation(Ex) 340 nm、 Emission(Em) 616 nm)
In the case of Cyanine
Cyanine is a fluorescent dye having fluorescence / excitation wavelength that is widely used in the biotechnology field. It can be used for fluorescent labeling of proteins and FACS.
Cyanine3 (Excitation(Ex) 550 nm、Emission(Em) 576 nm)
Cyanine5 (Excitation(Ex) 650 nm、Emission(Em) 684 nm)
Fluorescent beads lineup
※Please scroll horizontally.
|Fluorescent dye||Functional group||FF beads
Magnetic type・180±30 nm
Non-magnetic type・400±20 nm
|Europium||Carboxylic acid||FF Eu COOH beads
|FS Eu COOH beads
|Please contact us|
|Streptavidin||FF Eu Streptavidin beads
|FS Eu Streptavidin beads
|Protein G||FF Eu Protein G beads
|FS Eu Protein G beads
|Cyanine 3||Carboxylic acid||FF Cyanine3 COOH beads
|FS Cyanine3 COOH beads
|Streptavidin||FF Cyanine3 Streptavidin beads
|FS Cyanine3 Streptavidin beads
|Cyanine 5||Carboxylic acid||FF Cyanine5 COOH beads
|FS Cyanine5 COOH beads
|Streptavidin||FF Cyanine5 Streptavidin beads
|FS Cyanine5 Streptavidin beads
01. Sandwich immunoassay using FF Eu beads
This is an example of application to immunoassay by antibody immobilized FF beads. Since the FF beads contain plenty of fluorescent dye, the detection sensitivity of them has become higher than the labelled antibodies of the conventional immunoassay. In addition, the reaction efficiency and the measuring speed have been improved by using a magnet for antigen-antibody reaction.
Detection result of the α-fetoprotein (tumor marker) in human serum
02. Immunostaining using FF Eu beads
When staining of EGFR receptors in a xenograft specimen of A 431 human epidermoid carcinoma cell was performed by using FF Eu beads, the similar result to the result of the conventional immunostaining was obtained in a short time.
Immunostaining using FF beads
Sakamoto S, et al.Clinical Chemistry, 2014,60:4, 610–620,
- Papers /
- Related Products
- Screening by using ligand immobilized beads
- Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads
- Direct Quantification of Immobilized Proteins (Antibodies)
- Immobilization of antibodies on Protein A beads and Protein G beads
- Preparation of cell extract (Large scale method)
- Preparation of cell extract (Small scale method)
Development of an automatic magnetic immunostaining system for rapid intraoperative diagnosis of cancer metastasis
AIP Advances 10, 015106 (2020)
Development of an optimized dome-shaped magnet for rapid magnetic immunostaining
AIP Advances 10, 025317 (2020)
Smartphone based platform for real-time sharing of medical diagnostics information by optical detection of functionalized fluorescent magnetic nanoparticles
Biomed. Phys. Eng. Express, 5, 035014(2019)
Application of high-performance magnetic nanobeads to biological sensing devices.
Aal. Bioanal. Chem., doi: 10.1007/s00216-018-1548-y (2019).
Magnetically Promoted Rapid Immunofluorescence Staining for Frozen Tissue Sections
J. Histochem. Cytochem., Epub 2019 Apr 8
Development of magnet configurations for magnetic immunostaining
AIP ADVANCES 8, 056732 (2018)
Fast and sensitive medical diagnostic protocol based on integrating circular current lines for magnetic washing and optical detection of fluorescent magnetic nanobeads.
Sensing and Bio-Sensing Researc, 9, 7 (2016).
Magnetically Promoted Rapid Immunoreactions Using Functionalized Fluorescent Magnetic Beads:A Proof of Principle
Clinical Chemistry, 60:4 (2014).
- Please tell me how to separate FG beads (magnetic separation and centrifugation).
- Please tell me how to disperse FG beads (ultrasonic method and manual method).
- I mistakenly frozen some beads that were supposed to be stored in the refrigerator. Is it available?
- What is the number of particles per 1 mg of FS beads?
- What is the number of particles per 1 mg of FF beads?