Epoxy beads
Epoxy beads can immobilize NH2 groups such as proteins and phenolic OH groups.
Normal OH groups have a low reactivity, but phenolic OH groups have a high reactivity, so they can be immobilized on beads under alkaline conditions.
Magnetic beads
The lineup of this product is only regular FG beads.
Beads | FG beads |
---|---|
Code | TAS8848N1110 |
Price | Please contact us |
Storage conditions | 2-8 ℃ (no freezing), protect from light |
Storage buffer | Ultrapure water |
Magnetization | Superparamagnetism (≧10 emu/g) |
Size of beads | 180±30 nm |
Concentration | 20 mg/ml |
Functional groups | Epoxy groups |
Amounts of the functional groups | Approx. 250 nmol/mg of beads |
- Protocol
- SDS
- Papers /
Technical Information - Related Products
- FAQ
- Screening by using ligand immobilized beads
- Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads
- Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads (Small scale method)
- Competitive inhibition
- Drug elution
- Immobilization of Antibodies or Proteins on Epoxy Beads
- Direct Quantification of Immobilized Proteins (Antibodies)
- Preparation of cell extract (Large scale method)
- Preparation of cell extract (Small scale method)
Technical Information
Papers
-
Identification of RPL15 60S Ribosomal Protein as a Novel Topotecan Target Protein That Correlates with DAMP Secretion and Antitumor Immune Activation
J Immunol July 1, 2022, 209 (1) 171-179 -
Inhibition of adult T-cell leukemia cell proliferation by polymerized proanthocyanidin from blueberry leaves through JAK proteolysis
Cancer Science. 2022;113:1406–1416 -
K. Kobayashi-Nakamura et al. (2022)
Rhamnazin suppresses melanosome transport by promoting the ubiquitin-mediated proteasomal degradation of melanophilin
Journal of Dermatological Science 105 (2022) 45–54 -
Berberine is an insulin secretagogue targeting the KCNH6 potassium channel
NATURE COMMUNICATIONS(2021) 12:5616 -
Pesticide treatment reduces hydrophobic pollutant contamination in Cucurbita pepo through competitive binding to major latex-like proteins
Environmental Pollution 266 (2020) 115179 -
Factors regulating the differential uptake of persistent organic pollutants in cucurbits and non-cucurbits
Journal of Plant Physiology 245 (2020) 153094 -
Nobiletin and related polymethoxylated flavones bind to and inhibit the nuclear export factor Exportin-1 in NK leukemia cell line KHYG-1
Biochemical and Biophysical Research Communications 521 (2020) 457 -
Food-Derived Compounds Apigenin and Luteolin Modulate mRNA Splicing of Introns with Weak Splice Sites
iScience 22, 336–352, December 20, 2019 -
Uptake mechanisms of polychlorinated biphenyls in Cucurbita pepo via xylem sap containing major latex-like proteins
Environ. Exp. Bot., 162, 399(2019) -
Glabridin inhibits dexamethasone-induced muscle atrophy
Arch. Biochem. Biophys., 664, 157(2019) -
Morin suppresses cachexia-induced muscle wasting by binding to ribosomal protein S10 in carcinoma cells
Biochemical and Biophysical Research Communications 506 (2018) 773-779 -
Ribosomal protein S3 regulates XIAP expression independently of the NF-κB pathway in breast cancer cells
ONCOLOGY REPORTS, 38, 3205 (2017). -
The pleiotropic regulation of cyclin D1 by newly identified sesaminol-binding protein ANT2
Oncogenesis, 6, e311 (2017). -
Resveratrol directly targets DDX5 resulting in suppression of the mTORC1 pathway in prostate cancer
Cell Death Dis., 7, e2211 (2016). -
Disruption of Heat Shock Protein 90 (Hsp90)-Protein Kinase C (PKC ) Interaction by (-)-Maackiain Suppresses Histamine H1 Receptor Gene Transcription in HeLa Cells
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 290, NO. 45, pp. 27393 -
Mitochonic Acid 5 Binds Mitochondria and Ameliorates Renal Tubular and Cardiac Myocyte Damage
J. Am. Soc. Nephrol., DOI:10.1681 (2015). -
Flurbiprofen ameliorated obesity by attenuating leptin resistance induced by endoplasmic reticulum stress
EMBO Molecular Medicine, DOI: 10.1002 (2014). -
Apigenin sensitizes prostate cancer cells to Apo2L/TRAIL by targeting adenine nucleotide translocase-2
PLOS ONE, 8, e55922 (2013). -
A major latex-like protein is a key factor in crop contamination by persistent organic pollutants
Plant Physiology, 161, 2128 (2013). -
The Flavonoid Apigenin Downregulates CDK1 by DirectlyTargeting Ribosomal Protein S9
PLOS ONE, 8, e73219 (2013). -
Vesnarinone suppresses TNFα mRNA expression by inhibiting valosin-containing protein
Mol. Pharmacol., 83, 930 (2013). -
Identification of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) as a novel target of bisphenol A
PLOS ONE, 7, e50481 (2012). -
E. Okuda-Ashitaka et al. (2012)
Identification of NIPSNAP1 as a Nocistatin-interacting Protein Involving Pain Transmission
J. Biol. Chem., 287, 10403 (2012). -
Identification of Dynamin-2-mediated Endocytosis as a New Target of Osteoporosis Drugs, Bisphosphonates
Mol. Pharmacol., 77, 262 (2009). -
Mono-(2-ethylhexyl) phthalate Targets Glycogen Debranching Enzyme and Affects Glycogen Metabolism in Rat Testis
Toxicol. Sci., 109, 143 (2009). -
Atrazine binds to F1F0-ATP synthase and inhibits mitochondrial function in sperm
Biochem. Biophys. Res. Commun., 366, 66 (2008). -
High-performance affinity beads for identifying anti-NF-κB drug receptors
Methods Enzymol., 353, 81 (2002). -
High-performance affinity beads for identifying drug receptors
Nat. Biotechnol., 18, 877 (2000). -
Purification of membrane receptors with peptide-carrying affinity latex particles
Coll. Surf. B., 4, 231 (1995).
- Please tell me how to separate FG beads (magnetic separation and centrifugation).
- Please tell me how to disperse FG beads (ultrasonic method and manual method).
- I mistakenly frozen some beads that were supposed to be stored in the refrigerator. Is it available?
- What amount of the beads is required?
- What are the important points when designing a ligand?
- How are beads stored after the ligands are bound to them?
- Are there any methods other than HPLC for verifying whether or not ligand binding has been successful?
- How strong is the affinity for the proteins that are affinity purified?
- What is the purification efficiency?
- How is the cell extract prepared?
- Is there any problem with using frozen stock homogenate?
- How much protein supply is necessary?
- Can affinity purification be used with membrane proteins such as GPCRs and ion channels?
- There are many background bands. how can i reduce it?
- What should be done when a large number of bound protein bands are detected?
- Is it necessary to use the recommended buffer as the binding buffer?
- Why is it that both salt elution and boil elution are performed for elution?
- Does it happen that the band of bound protein becomes thin when the concentration of ligand is increased?
- Why can’t I see any bands of bound proteins?
- How long is the stable period of the ligand-immobilized beads?
- Is the optimal binding reaction time of 4 hours?
- What is the optimal bead type for immobiliding antibodies?
- Can I quantify the immobilization amount of the antibodies on the beads?
- What is the efficiency when immobiliding antibodies?
- Is there a way to increase the antibody immobilization efficiency (immobilization amount)?
- Can I disperse antibody-immobilized beads by ultrasonic device?
- When immobilizing antibodies to beads, the beads may adhere to the wall of the tube. Is there a way to suppress this?
- How can I improve dispersibility of antibodies immobilized beads?
- What is the optimal bead type for binding DNA?
- I want to analyze bound proteins with MS, but what should I do if the target protein band is thin?
- How much protein can be analyzed by MS?