NHS beads

Technical Information

• Catalog

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• FG winds issue 1

  Isolation of drug target protein ‐ Methotrexate
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• FG winds issue 3

  Isolation of drug target protein ‐ Bisindolylmaleimide
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• FG winds issue 4

  Isolation of drug target protein ‐ Vorinostat
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• Purification of proteins binding Bisindolylmaleimide X

  This study is the collaboration with a pharmaceutical company.
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Papers

• Yunhao Zhu et al.(2023)

  Acceleration of Protein Degradation by 20S Proteasome-Binding Peptides Generated by In Vitro Artificial Evolution
  Int. J. Mol. Sci. 2023, 24, 17486.

• N. Kajitani and M. Okada-Tsuchioka et al.(2023)

  G protein-biased LPAR1 agonism of prototypic antidepressants: Implication in the identification of novel therapeutic target for depression
  Neuropsychopharmacology; https://doi.org/10.1038/s41386-023-01727-9

• Xian-Yang Qin et al.(2023)

  Targeting transglutaminase 2 mediated exostosin glycosyltransferase 1 signaling in liver cancer stem cells with acyclic retinoid
  Cell Death & Disease volume 14, Article number: 358 (2023)

• Takahata et al.(2023)

  Immuno-Mass Spectrometry Workflow for Quantification of Serum α-Fetoprotein Using Antibody-Immobilized Magnetic Beads and Modified Eluents.
  Mass Spectrom (Tokyo). 2023;12(1):A0122.

• Suzuki et al.(2022)

  Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae
  Bio-potocol 12(9): e4403

• Watanabe et al.(2022)

  A chemoproteoinformatics approach demonstrates that aspirin increases sensitivity to MEK inhibition by directly binding to RPS5
  PNAS Nexus, 2022, 1, 1–11

• N. Iwamoto et al. (2022) [nSMOL]

  A rapid and universal liquid chromatograph-mass spectrometry-based platform, refmAb-Q nSMOL, for monitoring monoclonal antibody therapeutics
  Analyst, DOI: 10.1039/d2an01032a

• N. Iwamoto et al. (2022) [nSMOL]

  Structure-Indicated LC-MS/MS Bioanalysis of Therapeutic Antibodies
  Methods Mol Biol. 2022;2313:187-205

• Suzuki et al.(2021)

  A PX-BAR protein Mvp1/SNX8 and a dynamin-like GTPase Vps1 drive endosomal recycling
  eLife 2021;10:e69883.

• M. Ohuchi et al. (2021) [nSMOL]

  Use of an alternative signature peptide during development of a LC-MS/MS assay of plasma nivolumab levels applicable for multiple species
  Journal of Chromatography B 1162 (2021) 122489

• Zhang X et al. (2021) [ExoCounter]

  BATF2 prevents glioblastoma multiforme progression by inhibiting recruitment of myeloid-derived suppressor cells
  Oncogene 1516 (2021) 40

• Nomura et al.(2021)

  Stimulation of the Runx2 P1 promoter by collagen-derived dipeptide prolyl-hydroxyproline bound to Foxg1 and Foxo1 in osteoblasts
  Bioscience Reports (2021) 41 BSR20210304

• Takahashi et al.(2020)

  A Human DUB Protein Array for Clarification of Linkage Specificity of Polyubiquitin Chain and Application to Evaluation of Its Inhibitors
  Biomedicines 2020, 8, 152

• Takano et al.(2020)

  The Surprising Effect of Phenformin on Cutaneous Darkening and Characterization of Its Underlying Mechanism by a Forward Chemical Genetics Approach
  Int J Mol Sci. 2020 Feb 20;21(4):1451

• J. Cho et al. (2020)

  L-Arginine prevents cereblon-mediated ubiquitination of glucokinase and stimulates glucose-6-phosphate production in pancreatic β-cells
  Communications Biology (2020) 3:497

• K. Todoroki et al. (2020) [nSMOL]

  Bioanalytical methods for therapeutic monoclonal antibodies and antibody–drug conjugates: A review of recent advances and future perspectives
  Journal of Pharmaceutical and Biomedical Analysis 179 (2020) 112991

• L. Kong et al. (2020) [nSMOL]

  A novel LC-MS/MS approach to the pharmacokinetic study of free and bound aflibercept simultaneously
  Analytical and Bioanalytical Chemistry 412 (2020) 1003

• J. Cho et al. (2020)

  UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner
  Biochemical and Biophysical Research Communications 527 (2020) 668

• Meguro et al.(2020)

  Pex3 confines pexophagy receptor activity of Atg36 to peroxisomes by regulating Hrr25-mediated phosphorylation and proteasomal degradation
  J. Biol. Chem. (2020) 295(48) 16292–16298

• K. Akiyoshi et al. (2020)

  Wash-free detection of biological target using cluster formation of magnetic markers
  Journal of Magnetism and Magnetic Materials 500 (2020) 166356

• Mochida et al.(2020)

  Super-assembly of ER-phagy receptor Atg40 induces local ER remodeling at contacts with forming autophagosomal membranes
  NATURE COMMUNICATIONS (2020) 11:3306

• Suzuki et al.(2019)

  A bipartite sorting signal ensures specificity of retromer complex in membrane protein recycling
  J. Cell Biol. 2019 Vol. 218 No. 9 2876–2886

• Nomura et al.(2019)

  Collagen-derived dipeptide prolyl hydroxyproline directly binds to Foxg1 to change its conformation and inhibit the interaction with Runx2
  Bioscience, Biotechnology, and Biochemistry, Volume 83, Issue 11, 2 November 2019, Pages 2027–2033

• Y. Uchihara et al. (2019)

  Methotrexate significantly induces apoptosis by inhibiting STAT3 activation in NPM-ALK-positive ALCL cells
  Biochemical Pharmacology, 170 (2019) 113666

• N. Iwamoto et al. (2019) [nSMOL]

  Acceleration of nano-surface and molecular-orientation limited(nSMOL) proteolysis with acidified reduction pretreatment forquantification of Tocilizumab
  Journal of Pharmaceutical and Biomedical Analysis 164 (2019) 467–474

• N. Iwamoto et al. (2019) [nSMOL]

  Comparison of Bevacizumab Quantification Results in Plasma of Non-small Cell Lung Cancer Patients Using Bioanalytical Techniques Between LC-MS/MS, ELISA, and Microfluidic-based Immunoassay
  The AAPS Journal November 2019, 21:101

• N. Iwamoto et al. (2019) [nSMOL]

  Multiplexed monitoring of therapeutic antibodies for inflammatory diseases using Fab-selective proteolysis nSMOL coupled with LC-MS.
  J. Immunol. Methods, 472, 44(2019)

• N. Iwamoto et al. (2019) [nSMOL]

  Regulated LC-MS/MS bioanalysis technology for therapeutic antibodies and Fc-fusion proteins using structure-indicated approach
  Drug Metabolism and Pharmacokinetics 34, 19 (2019)

• Y. Kabe et al. (2019) [ExoCounter]

  Application of high-performance magnetic nanobeads to biological sensing devices.
  Aal. Bioanal. Chem., doi: 10.1007/s00216-018-1548-y (2019).

• K. Harada et al. (2019)

  Two distinct mechanisms target the autophagy-related E3 complex to the pre-autophagosomal structure
  Elife, 8, e43088(2019)

• Suzuki et al.(2018)

  Development of a fluorescent peptide for the highly sensitive and selective detection of vascular endothelial growth factor
  Sensors and Actuators B 276 (2018) 230–237

• N. Iwamoto et al. (2018) [nSMOL]

  Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis
  Analytical Biochemistry 540–541 (2018) 30–37

• Sekiguchi et al.(2018)

  MHC-associated peptide proteomics enabling highly sensitive detection of immunogenic sequences for the development of therapeutic antibodies with low immunogenicity
  MABS 2018, VOL. 10, NO. 8, 1168–1181

• K. Enpuku et al. (2018)

  Wash-free detection of C-reactive protein based on third-harmonic signal measurement of magnetic markers.
  Jpn. J. Appl. Phys., 57, 112, 090309 (2018).

• N. Iwamoto et al. (2018) [nSMOL]

  Recent advances in mass spectrometry-based approaches for proteomics and biologics: Great contribution for developing therapeutic antibodies
  Pharmacology and Therapeutics 185, 147 (2018)

• Y. Kabe et al. (2018) [ExoCounter]

  Development of a Highly Sensitive Device for Counting the Number of Disease-Specific Exosomes in Human Sera
  Clinical Chemistry, DOI: 10.1373/clinchem.2018.291963 (2018).

• T. Kotani et al. (2018)

  The Atg2-Atg18 complex tethers pre-autophagosomal membranes to the endoplasmic reticulum for autophagosome formation
  Proc. Natl. Acad. Sci. USA, 115, 10363(2018)

• Uematsu et al.(2017)

  Accumulation of undegraded autophagosomes by expression of dominant-negative STX17 (syntaxin 17) mutants
  Autophagy, 13:8 (2017), 1452-1464

• Nishimura et al.(2017)

  Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains
  The EMBO Journal (2017)36:1719-1735

• N. Iwamoto et al. (2017) [nSMOL]

  LC–MS bioanalysis of Trastuzumab and released emtansine usingnano-surface and molecular-orientation limited (nSMOL) proteolysisand liquid–liquid partition in plasma of Trastuzumabemtansine-treated breast cancer patients
  Journal of Pharmaceutical and Biomedical Analysis 145 (2017) 33–39

• Y. Inoue et al. (2017)

  Quenched Electrochemiluminescence Imaging using Electro-Generated Substrate for Sensitive Detection of Catalase as Potential Enzyme Reporter System.
  Electrochimica Acta., 240, 447 (2017)

• M. Tsushima et al. (2017)

  Selective purification and chemical labeling of a target protein on ruthenium photocatalystfunctionalized affinity beads
  Chem. Commun., 53, 4838 (2017).

• N. Iwamoto et al. (2016) [nSMOL]

  Validated LC–MS/MS analysis of immune checkpoint inhibitorNivolumab in human plasma using a Fab peptide-selectivequantitation method: nano-surface and molecular-orientation limited(nSMOL) proteolysis
  Journal of Chromatography B, 1023-1024 (2016) 9–16

• L. Jia et al. (2016)

  Synergistic degradation of arabinoxylan by free and immobilized xylanases and arabinofuranosidase
  Biochem. Eng. J., 114, 268 (2016).

• N. Iwamoto et al. (2016) [nSMOL]

  Application of nano-surface and molecular-orientation limited proteolysis to LC?MS bioanalysis of cetuximab.
  Bioanalysis, 8, 1009 (2016).

• N. Iwamoto et al. (2016) [nSMOL]

  Fully validated LCMS bioanalysis of Bevacizumab in human plasma using nano-surface and molecular-orientation limited (nSMOL) proteolysis
  Drug Metab. Pharmacokinet., 31, 46 (2016).

• K. Ishii, Y.Sugimura. (2015)

  Identification of a new pharmacological activity of the phenylpiperazine derivative naftopidil:tubulin-binding drug
  J. Chem. Biol., 8, 5 (2015).

• Y. Suzuki, A. Kuno, Y. Chiba. (2015)

  Development of fluorescent probes for “On-Off” switching baseddetection of lectin?saccharide interactions.
  Sens. Actuators B., 220, 389 (2015).

• K. Takahashi et al. (2014)

  Functional Analysis of Light-harvesting-like Protein 3 (LIL3) and Its Lightharvesting Chlorophyll-binding Motif in Arabidopsis
  J. Biol. Chem., 289, 987 (2014).

• N. Iwamoto et al. (2014) [nSMOL]

  Selective detection of complementaritydetermining regions of monoclonal antibody by limiting protease access to the substrate: nanosurface and molecular-orientation limited proteolysis

  Analyst, 139, 576 (2014).