Buffer

For FG beads only

Buffer

No need for adjustment or dilution !!

We sell buffers for FG beads used in the protocol.
You don’t have to prepare the buffer yourself, you can start experimenting right away.
The buffer has a lineup of kits and individual items required for each protocol.

※Please scroll horizontally.

Products Code Corresponding protocol Capacity Usage or content
105
Immobilization of Antibodies or Proteins on NHS beads
011
Immunoprecipitation
001
Screening by using ligand immobilized beads
Buffer kit

Antibody immobilization Buffer Kit

  • 25 mM MES-NaOH(pH6.0) 3 mL
  • Protein immobilized beads wash/storage buffer 1)45 mL
  • 1 M Aminoethanol(pH8.0) 15 mL
  • 10%(w/v) NP-40 0.2 mL
TAB1200N0310 Kit for 50 samples of antibody immobilization operation

Protein immobilization Buffer Kit

  • 25 mM HEPES-NaOH(pH7.0) 3 mL
  • Protein immobilized beads wash/storage buffer 1)45 mL
  • 1 M Aminoethanol(pH8.0) 15 mL
  • 10%(w/v) NP-40 0.2 mL
TAB1200N0319 Kit for 50 samples of protein immobilization operation

IP Buffer Kit

  • 150 mM KCl buffer, PMSF(-) 2) 75 ml
  • 0.1 M Glycine-HCl(pH2.5) 1.5 mL
  • 1 M Tris-HCl(pH9.0) 0.1 mL
TAB1200N0320 Kit for 50 samples of immunoprecipitation operation

Screening Buffer Kit

  • 100 mM KCl buffer, DTT(-), PMSF(-) 3)75 mL
  • 1 M KCl buffer , DTT(-), PMSF(-) 4)2 mL
TAB1200N0330 Kit for 50 samples of affinity purification
Individual buffer
25 mM MES-NaOH(pH6.0) TAB1200N0311 100 ml Antibody immobilized buffer
25 mM HEPES-NaOH(pH7.0) TAB1200N0312 100 ml Protein immobilization buffer
Protein immobilized beads wash/storage buffer 1) TAB1200N0313 100 ml Wash / storage buffer
1 M Aminoethanol(pH8.0) TAB1200N0314 100 ml Masking buffer
150 mM KCl buffer, PMSF(-) 2) TAB1200N0321 100 ml Binding / washing buffer
0.1 M Glycine-HCl(pH2.5) TAB1200N0322 100 ml Acid elution buffer
1 M Tris-HCl(pH9.0) TAB1200N0323 100 ml Neutralizing buffer
100 mM KCl buffer, DTT(-),PMSF(-) 3) TAB1200N0331 100 ml Binding / washing buffer
1 M KCl buffer , DTT(-), PMSF(-) 4) TAB1200N0332 100 ml Salt elution buffer
1 M HEPES-NaOH(pH7.9) TAB1200N0911 50 ml Solution for preparing various buffers
2.5 M KCl TAB1200N0912 100 ml Solution for preparing various buffers
1 M MgCl2 TAB1200N0913 100 ml Solution for preparing various buffers
1 M CaCl2 TAB1200N0914 100 ml Solution for preparing various buffers
10%(w/v) NP-40 TAB1200N0915 100 ml Solution for preparing various buffers

1) Composition10 mM HEPES-NaOH(pH7.9), 50 mM KCl, 1 mM EDTA, 10%(v/v) glycerol

2) Composition20 mM HEPES-NaOH(pH7.9), 150 mM KCl, 1 mM MgCl2, 0.2 mM CaCl2, 0.2 mM EDTA, 10%(v/v) glycerol, 0.1%(w/v) NP-40
※Just before use, add PMSF to a final consentration of 0.2 mM

3) Composition20 mM HEPES-NaOH(pH7.9), 100 mM KCl, 1 mM MgCl2, 0.2 mM CaCl2, 0.2 mM EDTA, 10%(v/v) glycerol, 0.1%(w/v) NP-40
※Just before use, add DTT to a final concentration of 1 mM and PMSF to a final consentration of 0.2 mM

4) Composition20 mM HEPES-NaOH(pH7.9), 1 M KCl, 1 mM MgCl2, 0.2 mM CaCl2, 0.2 mM EDTA, 10%(v/v) glycerol, 0.1%(w/v) NP-40
※Just before use, add DTT to a final concentration of 1 mM and PMSF to a final consentration of 0.2 mM

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