The nSMOLTMmethod, developed by Shimadzu Corporation, is an epoch-making technology capable of selectively proteolyzingthe Fab region of monoclonal antibodies.
Using the “nSMOL™ Antibody BA Kit”, monoclonal antibodies can be recovered from biological samples such as blood, and only the Fab region can be proteolyzed.
By digesting only the Fab region, sample complexity is reduced, and FG beads Trypsin DARTTM is used, so there is no trypsin contamination.
It can be applied without depending on antibody drugs or reference antibodies.
Exosomes in the samples can be directly quantified with these ELISA kits without any purification processes.
Exosomes are captured by immobilized monoclonal antibodies against CD9, CD63 or CD81, and then detected with antibodies against CD9, CD63 or CD81 conjugated with horse radish peroxidase.
You can measure with the popular plate readers without any special apparatuses.
Recombinant CD9, CD63 or CD81 proteins are fixed on the surface of FS Eubeads and utilized as standard reagents instead of exosomes in order to guarantee the stability and reproducibility.
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