There are many background bands. how can i reduce it?
It is necessary to consider the optimal conditions by varying the concentration of ligand binding to the FG beads, the salt concentration in the buffer, and the surfactant concentration. This can sometimes be improved by carefully performing dispersion when screening. It is also important to centrifuge the protein solution before use to remove the insoluble fraction. Insufficient masking is also a possibility, so it is necessary to suitably mask the functional groups which are not bound by the ligands.
