Contracted services
We provide contracted services for each step, or all steps, of ligand design, immobilization, and affinity purification. We are also conducting comparative experiments using different types of beads.
Flow and examples of contracted services
STEP 1. Ligand design and synthesis
If the compound does not have a functional group that is effective for immobilization on FG beads, a functional group that is effective for immobilization is introduced into the compound.
（Example）
STEP 2. Immobilize the ligand on FG beads.
The ligand is immobilized on FG beads, and the amount of immobilization is quantified or the immobilization confirmation experiment is performed.
（Example）
STEP 3. Purification of ligand-binding protein
Affinity purification is performed using beads with immobilized ligand and Crude cell extract (protein mixture). Depending on the situation, we will narrow down the target proteins by performing competitive inhibition and drug elution.
（Example）
Please contact us for the details of the contracted service.
- Protocol
- SDS
- Papers /
Technical Information - Related Products
- FAQ
- Screening by using ligand immobilized beads
- Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads
- Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads (Small scale method)
- Immobilization of ligands (carboxylic compounds) on OH beads
- Immobilization of ligands (carboxylic compounds) on OH beads (Small scale method)
- Immobilization of ligands (carboxylic compounds) on NH2 beads
- Immobilization of ligands (carboxylic compounds) on NH2 beads (Small scale method)
- Immobilization of ligands (compounds with NH2 groups) on COOH beads
- Immobilization of ligands (compounds with NH2 groups) on COOH beads (Small scale method)
- Competitive inhibition
- Drug elution
- Immobilization of ligands (compounds with NH2 groups) on NHS beads
- Immobilization of ligands (compounds with NH2 groups) on NHS beads (Small scale method)
- Immobilization of ligands (compounds with OH groups) on COOH beads
- Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads
- Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction
- Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction
- Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction (Small scale method)
- Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography)
- Immunoprecipitation
- Immobilization of proteins on COOH beads
- Immobilization of His-Tag proteins on Ts beads
- Immobilization of Antibodies or Proteins on NHS beads
- Immobilization of Antibodies or Proteins on Epoxy Beads
- Direct Quantification of Immobilized Proteins (Antibodies)
- Immobilization of antibodies on Protein A beads and Protein G beads
- Immobilization of double strand DNA on Plain beads
Technical Information
- Is it possible to manufacture beads with particle sizes other than those in the lineup?
- What is the general process used to bind ligands to the beads?
- What amount of the beads is required?
- What are the important points when designing a ligand?
- Is it possible to bind secondary amines?
- Are there any methods other than HPLC for verifying whether or not ligand binding has been successful?
- What is the purification efficiency?
- What is the optimal bead type for binding proteins?
- What is the efficiency when binding proteins?
- How is the cell extract prepared?
- Is there any problem with using frozen stock homogenate?
- How much protein supply is necessary?
- What should be done when a large number of bound protein bands are detected?
- Is it necessary to use the recommended buffer as the binding buffer?
- Why is it that both salt elution and boil elution are performed for elution?
- Does it happen that the band of bound protein becomes thin when the concentration of ligand is increased?
- Why can’t I see any bands of bound proteins?
- Is the optimal binding reaction time of 4 hours?
- What is the optimal bead type for immobiliding antibodies?
- Can I quantify the immobilization amount of the antibodies on the beads?
- What is the efficiency when immobiliding antibodies?
- What is the optimal bead type for binding DNA?
- What method is used to bind RNA？
- I want to analyze bound proteins with MS, but what should I do if the target protein band is thin?
- How much protein can be analyzed by MS?
