When immobilizing antibodies to beads, the beads may adhere to the wall of the tube. Is there a way to suppress this?
It may be improved by the following methods.
-Use a protein low adsorption tube.
-Agitate with a microtube mixer instead of overturning and mixing.
-After the immobilization reaction, the beads are dispersed by manual agitation and centrifugal (magnetic) separation is performed.