When immobilizing antibodies to beads, the beads may adhere to the wall of the tube. Is there a way to suppress this?

It may be improved by the following methods.

-Use a protein low adsorption tube.

-Agitate with a microtube mixer instead of overturning and mixing.

-After the immobilization reaction, the beads are dispersed by manual agitation and centrifugal (magnetic) separation is performed.

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