COOH beads
COOH beads can immobilize compounds, antibodies or proteins with NH2 groups directly on the beads.
COOH beads are used in a wide range of applications such as chemical biology, immunoprecipitation and cell separation.
Magnetic beads
The lineup of this product is only regular FG beads.
| Beads | FG beads |
|---|---|
| Code | TAS8848N1140 |
| Price | Please contact us |
| Storage conditions | 2-8 ℃ (no freezing) |
| Storage buffer | Ultrapure water |
| Magnetization | Superparamagnetism (≧10 emu/g) |
| Size of beads | 180±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Carboxyl group |
| Amounts of the functional groups | Approx. 250 nmol/mg of beads |
Fluorescent beads
Fluorescent beads contain fluorescent dyes, and there are FF beads with magnetic material and non-magnetic FS beads.
| Beads | FF beads （Magnetic type） |
FS beads （Non-magnetic type） |
||
|---|---|---|---|---|
| Code | Fluorescent dye | Europium | TAB8849N2140 | TAB5849N2140 |
| Cyanine 3 | TAB8850N2140 | TAB5850N2140 | ||
| Cyanine 5 | TAB8851N2140 | TAB5851N2140 | ||
| Price | Please contact us | |||
| Storage conditions | 2-8 ℃ (no freezing), protect from light | |||
| Storage buffer | Ultrapure water | |||
| Magnetization | Superparamagnetism (≧10 emu/g) | No magnetization | ||
| Size of beads | 180±30 nm | 400±20 nm | ||
| Concentration | 10 mg/mL | |||
| Functional groups | Carboxyl group | |||
| Amounts of the functional groups | Approx. 200 nmol/mg of beads | |||
- Protocol
- SDS
- Papers /
Technical Information - Related Products
- FAQ
- Screening by using ligand immobilized beads
- Immobilization of ligands (compounds with NH2 groups) on COOH beads
- Immobilization of ligands (compounds with NH2 groups) on COOH beads (Small scale method)
- Competitive inhibition
- Drug elution
- Immobilization of ligands (compounds with OH groups) on COOH beads
- Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography)
- Immunoprecipitation
- Immobilization of proteins on COOH beads
- Direct Quantification of Immobilized Proteins (Antibodies)
- Preparation of cell extract (Large scale method)
- Preparation of cell extract (Small scale method)
Technical Information
Papers
-
Activation of AMP-activated protein kinase (AMPK)
iScience 26, 106293, April 21, 2023 -
Azithromycin, a potent autophagy inhibitor for cancer therapy, perturbs cytoskeletal protein dynamics
British Journal of Cancer volume 128, pages1838–1849 (2023) -
Intelectin1 ameliorates macrophage activation via inhibiting the nuclear factor kappa B pathway
Endocrine Journal 69 (2022) 5 Pages 539-546 -
Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol
COMMUNICATIONS BIOLOGY (2022) 5:564 -
Nan Yagishita-Kyo et al. (2021)
Testosterone interrupts binding of Neurexin and Neuroligin that are expressed in a highly socialized rodent, Octodon degus
Biochemical and Biophysical Research Communications 551 (2021) 54 -
TORC1 inactivation stimulates autophagy of nucleoporin and nuclear pore complexes
Journal of Cell Biology 2020 Vol. 219 No. 7 e201910063 -
Tsujita et al.(2013) [ExoCounter]
Ultrahigh-Sensitivity Biomarker Sensing System Based on the Combination of Optical Disc Technologies and Nanobead Technologies
Japanese Journal of Applied Physics 52 (2013) 09LB02 -
Salicylic Acid Induces Mitochondrial Injury by Inhibiting Ferrochelatase Heme Biosynthesis Activity
Mol. Pharmacol., DOI:10.1124 (2013). -
Synthesis and applications of magnetic nanoparticles for biorecognition and point of care medical diagnostics
Nanotechnology, 21, 442001 (2010). -
Capsaicin binds to prohibitin 2 and displaces it from the mitochondria to the nucleus
Biochem. Biophys. Res. Commun., 379, 519 (2009). -
Development of a chemical screening system using aqueorin-fused protein
Biochem. Biophys. Res. Commun., 368, 600 (2008). -
A new mechanism of methotrexate action revealed by target screening with affinity beads
Mol. Pharmacol., 70, 1832 (2006).
- Please tell me how to separate FG beads (magnetic separation and centrifugation).
- Please tell me how to disperse FG beads (ultrasonic method and manual method).
- I mistakenly frozen some beads that were supposed to be stored in the refrigerator. Is it available?
- What amount of the beads is required?
- What are the important points when designing a ligand?
- Is it possible to bind secondary amines?
- How are beads stored after the ligands are bound to them?
- Are there any methods other than HPLC for verifying whether or not ligand binding has been successful?
- How strong is the affinity for the proteins that are affinity purified?
- What is the purification efficiency?
- What is the optimal bead type for binding proteins?
- When binding proteins, what should be done if there is lysine residue at a location related to binding with the target substance?
- What is the efficiency when binding proteins?
- What is the optimal bead type for binding peptides?
- How is the cell extract prepared?
- Is there any problem with using frozen stock homogenate?
- How much protein supply is necessary?
- Can affinity purification be used with membrane proteins such as GPCRs and ion channels?
- There are many background bands. how can i reduce it?
- What should be done when a large number of bound protein bands are detected?
- Is it necessary to use the recommended buffer as the binding buffer?
- Why is it that both salt elution and boil elution are performed for elution?
- Does it happen that the band of bound protein becomes thin when the concentration of ligand is increased?
- Why can’t I see any bands of bound proteins?
- How long is the stable period of the ligand-immobilized beads?
- Is the optimal binding reaction time of 4 hours?
- What is the optimal bead type for immobiliding antibodies?
- Can I quantify the immobilization amount of the antibodies on the beads?
- What is the efficiency when immobiliding antibodies?
- Is there a way to increase the antibody immobilization efficiency (immobilization amount)?
- Can I disperse antibody-immobilized beads by ultrasonic device?
- When immobilizing antibodies to beads, the beads may adhere to the wall of the tube. Is there a way to suppress this?
- How can I improve dispersibility of antibodies immobilized beads?
- I want to analyze bound proteins with MS, but what should I do if the target protein band is thin?
- How much protein can be analyzed by MS?
