Is there a way to increase the antibody immobilization efficiency (immobilization amount)?

There are several ways. Please refer to the following.

(1) In the case of NHS beads, the amount of immobilization may increase by lengthening the immobilization reaction time. However, the NHS group is susceptible to hydrolysis and causes non-specific adsorption, so the reaction time should be within 6 hours.

(2) In the case of NHS beads, the immobilization reaction may be hindered by a composition other than the recommended immobilization buffer. If the antibody storage solution contains additives such as salts or glycerol, buffer substitution will improve the immobilization efficiency.

(3) The amount of immobilization increases by increasing the amount of antibody added during the immobilization reaction.

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