Protein A beads
Protein A beads can mainly immobilize antibodies (IgG) on beads by affinity purification.
It can be used for antibody purification and immunoprecipitation experiments using antigen-antibody reaction.
Protein A is a protein derived from the cell wall of Staphylococcus aureus (S. aureus).

 
    Magnetic beads
The product lineup includes regular FG beads and highly magnetically responsive HM beads.
| Beads | FG beads | HM beads (High Magnetic Response Type) | 
|---|---|---|
| Code | TAS8848N1172 | TAB8848N3172 | 
| Price | Please contact us | |
| Storage conditions | 2-8 ℃ (no freezing) | |
| Storage buffer | 10 mM HEPES(pH7.9), 50 mM KCl, 1 mM EDTA, 10% glycerol | 10 mM HEPES(pH7.9) | 
| Magnetization | Superparamagnetism (≧10 emu/g) | Superparamagnetism (≧20 emu/g) | 
| Size of beads | 180±30 nm | 140±20 nm | 
| Concentration | 20 mg/ml | |
| Functional groups | Protein A | |
| Binding ability | ≧35 ug Human IgG/mg of beads | |
Binding properties of Protein A and Protein G to IgG
※Please scroll horizontally.
| Animal species | Human | Mouse | ||||||
|---|---|---|---|---|---|---|---|---|
| Subclass | IgG1 | IgG2 | IgG3 | IgG4 | IgG1 | IgG2a | IgG2b | IgG3 | 
| Protein A | ◎ | ◎ | △ | ◎ | △ | ◎ | ◎ | ◎ | 
| Protein G | ◎ | ◎ | ◎ | ◎ | ◎ | ◎ | ◎ | ◎ | 
| Animal species | Rat | Bovine | Chicken | Rabbit | Horse | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Subclass | IgG1 | IgG2a | IgG2b | IgG2c | IgG1 | IgG2 | IgG | IgY | IgG | IgG | 
| Protein A | 〇 | × | × | ◎ | × | ◎ | × | × | ◎ | △ | 
| Protein G | 〇 | ◎ | 〇 | ◎ | ◎ | ◎ | × | × | ◎ | ◎ | 
| Animal species | Goat | Guinea pig | Dog | Cat | Hamster | Sheep | Pig | ||
|---|---|---|---|---|---|---|---|---|---|
| Subclass | IgG1 | IgG2 | IgG | IgG | IgG | IgG | IgG1 | IgG2 | IgG | 
| Protein A | × | ◎ | ◎ | ◎ | ◎ | △ | × | ◎ | ◎ | 
| Protein G | ◎ | ◎ | 〇 | ◎ | 〇 | – | ◎ | ◎ | ◎ | 
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 Technical Information
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Technical Information
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		Immunoprecipitation(1) 
 Learn More→
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		Immunoprecipitation(2) 
 Learn More→
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		Immunoprecipitation (3) 
 Learn More→
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		Immunoprecipitation (4) 
 Learn More→
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		Chromatin immunoprecipitation 1 unichi Yamamoto, Tokyo Medical University 
 Learn More→
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		Chromatin immunoprecipitation 2 obuo Horikoshi, Houston Methodist Research Institute Weill Cornell Medical College 
 Learn More→
papers
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		Tumor Suppressive Role of the PRELP Gene in Ovarian Clear Cell Carcinoma 
 J. Pers. Med. 2022, 12(12), 1999; https://doi.org/10.3390/jpm12121999
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		An oncogenic splice variant of PDGFRα in adult glioblastoma as a therapeutic target for selective CDK4/6 inhibitors 
 Scientifc Reports (2022) 12:1275
- 
		
		α1,4- Linked N- acetylglucosamine suppresses gastric cancer development by inhibiting Mucin-1-mediated signaling. 
 Cancer Science. 2022;00:1–12
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		Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol 
 COMMUNICATIONS BIOLOGY (2022) 5:564
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		Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules 
 INTERNATIONAL JOURNAL OF ONCOLOGY 60: 4, 2022
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		Identification of the mutation signature of the cancer genome caused by irradiation 
 Radiotherapy and Oncology 155 (2021) 10
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		Exploiting ubiquitin ligase cereblon as a target for small-molecule compounds in medicine and chemical biology 
 Cell Chemical Biology 28, July 15, 2021
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		N-glycosylation of Rim21 at an unconventional site fine-tunes its behavior in the plasma membrane 
 Cell Structure and Function 2020, https://doi.org/10.1247/csf.19021
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		Artificial switching of the metabolic processing pathway of an etiologic factor, β2-microglobulin, by a “navigator” molecule 
 Journal of Controlled Release 327 (2020) 8–18
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		Mismatch repair dependence of replication stress-associated DSB recognition and repair 
 Heliyon 5 (2019) e03057
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		ISGF3 with reduced phosphorylation is associated with constitutive expression of interferon-induced genes in aging cells 
 npj Aging and Mechanisms of Disease (2018)4:11
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		A Novel Autoantibody against Plexin D1 in Patients with Neuropathic Pain 
 Ann. Neurol., 84, 200 (2018).
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		Transport Granules Bound with Nuclear Cap Binding Protein and Exon Junction Complex Are Associated with Microtubules and Spatially Separated from eIF4E Granules and P Bodies in Human Neuronal Processes 
 Front. Mol. Biosci. 4:93. doi: 10.3389/fmolb.2017.00093
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		A proteomics method using immunoaffinity fluorogenic derivatization-liquid chromatography/tandem mass spectrometry (FD‐LC‐MS/MS) to identify a set of interacting proteins. 
 Biomedical Chromatography, DOI: 10.1002/bmc.4063 (2017).
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		Xeroderma pigmentosum group C protein interacts with histones: regulation by acetylated states of histone H3 
 Genes to Cells (2017) 22, 310–327
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		TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells 
 NATURE COMMUNICATIONS 8: 1592
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		N-Myristoylation of the Rpt2 subunit of the yeast 26S proteasome is implicated in the subcellular compartment?specific protein quality control system 
 Journal of Proteomics, 130, 33 (2016)
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		Development of an efficient entire-capsid-coding-region amplification method for direct detection of poliovirus from stool extracts 
 J. Clin. Microbiol., 53, 73 (2015).
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		Opt2 mediates the exposure of phospholipids during cellular adaptation to altered lipid asymmetry 
 J Cell Sci (2015) 128 (1): 61–69.
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		Identification of the Specific Interactors of the Human Lariat RNA Debranching Enzyme 1 Protein 
 Int. J. Mol. Sci., 16, 3705 (2015).
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		DNA Methylation Restricts Lineage-specific Functions of Transcription Factor Gata4 during Embryonic Stem Cell Differentiation 
 PLOS Genetics, 9, e1003574 (2013).
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		Development of poliovirus extraction method from the stool extracts by using magnetic nanoparticles sensitized with soluble poliovirus receptor. 
 J. Clin. Microbiol. DOI:10.1128 (2013).
- Please tell me how to separate FG beads (magnetic separation and centrifugation).
- Please tell me how to disperse FG beads (ultrasonic method and manual method).
- I mistakenly frozen some beads that were supposed to be stored in the refrigerator. Is it available?
- How is the cell extract prepared?
- Is there any problem with using frozen stock homogenate?
- How much protein supply is necessary?
- There are many background bands. how can i reduce it?
- What should be done when a large number of bound protein bands are detected?
- Is it necessary to use the recommended buffer as the binding buffer?
- Does it happen that the band of bound protein becomes thin when the concentration of ligand is increased?
- Why can’t I see any bands of bound proteins?
- How long is the stable period of the ligand-immobilized beads?
- What is the optimal bead type for immobiliding antibodies?
- Can I quantify the immobilization amount of the antibodies on the beads?
- What is the efficiency when immobiliding antibodies?
- Is there a way to increase the antibody immobilization efficiency (immobilization amount)?
- Can I disperse antibody-immobilized beads by ultrasonic device?
- When immobilizing antibodies to beads, the beads may adhere to the wall of the tube. Is there a way to suppress this?
- How can I improve dispersibility of antibodies immobilized beads?
- I want to analyze bound proteins with MS, but what should I do if the target protein band is thin?
- How much protein can be analyzed by MS?
