At present there are 13 types of FG beads®, which can be selected according to the functional group of the substance that will be bound.
With affinity purification, it is necessary to optimize the amount of the compound which is immobilized on the FG beads®. If the amount immobilized is too large, steric hindrance may impair binding to the target protein, or the surface may become hydrophobic, resulting in larger amounts of non-specific protein adsorption. It is possible to change the amount immobilized on the beads by varying the concentration of the substance during the immobilizing reaction.
For low molecular weight compounds with a known structure-activity relationship, locations which are not related to the activity can be used for binding to the FG beads®. For low molecular weight compounds with an unknown structure-activity relationship, immobiize the compound on the FG beads® at several available locations and perform affinity purification of the proteins that bind to each. Then check whether or not the affinity-purified protein is related to drug efficacy.
・NeutrAvidin™ is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.