We offers protocols about immobilization of ligands and purification of proteins etc.
No. | Protocol | File |
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001 | Screening by using ligand immobilized beads |
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003 | Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads |
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004 | Immobilization of ligands (carboxylic compounds) on OH beads |
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005 | Immobilization of ligands (carboxylic compounds) on NH2 beads |
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008 | Immobilization of ligands (compounds with NH2 groups) on COOH beads |
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012 | Competitive inhibition |
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013 | Drug elution |
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014 | Immobilization of ligands (compounds with NH2 groups) on NHS beads |
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108 | Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads |
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109 | Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction |
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111 | Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction |
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201 | Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) |
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No. | Protocol | File |
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003S | mmobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads |
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004S | Immobilization of ligands (carboxylic compounds) on OH beads |
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005S | Immobilization of ligands (carboxylic compounds) on NH2 beads |
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008S | Immobilization of ligands (compounds with NH2 groups) on COOH beads化 |
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014S | Immobilization of ligands (compounds with NH2 groups) on NHS beads |
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015S | Immobilization of MTX aminated derivatives on NHS beads |
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109S | Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction |
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111S | Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction |
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No. | Protocol | File |
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011 | Immunoprecipitation |
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101 | Immobilization of proteins on COOH beads |
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102 | Immobilization of His-Tag proteins on Ts beads |
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105 | Immobilization of Antibodies or Proteins on NHS beads |
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106 | Immobilization of Antibodies or Proteins on Epoxy Beads |
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107 | Direct Quantification of Immobilized Proteins (Antibodies) |
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108 | Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads |
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110 | Immobilization of antibodies on Protein A beads and Protein G beads |
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No. | Protocol | File |
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001 | Screening by using ligand immobilized beads |
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301 | Immobilization of double strand DNA on Plain beads |
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No. | Protocol | File |
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401 | Preparation of cell extract (Large scale method) |
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402 | Preparation of cell extract (Small scale method) |
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