We offers protocols about immobilization of ligands and purification of proteins etc.

Chemical pulldown

No.	Protocol	File
001	Screening by using ligand immobilized beads	201KB
003	Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads	90KB
004	Immobilization of ligands (carboxylic compounds) on OH beads	67KB
005	Immobilization of ligands (carboxylic compounds) on NH2 beads	95KB
008	Immobilization of ligands (compounds with NH2 groups) on COOH beads	133KB
012	Competitive inhibition	103KB
013	Drug elution	101KB
014	Immobilization of ligands (compounds with NH2 groups) on NHS beads	88KB
108	Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads	89KB
109	Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction	74KB
111	Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction	70KB
201	Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography)	2,345KB

No.	Protocol	File
003S	mmobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads	90KB
004S	Immobilization of ligands (carboxylic compounds) on OH beads	67KB
005S	Immobilization of ligands (carboxylic compounds) on NH2 beads	95KB
008S	Immobilization of ligands (compounds with NH2 groups) on COOH beads化	133KB
014S	Immobilization of ligands (compounds with NH2 groups) on NHS beads	88KB
015S	Immobilization of MTX aminated derivatives on NHS beads	70KB
109S	Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction	244KB
111S	Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction	244KB

Immunoprecipitation、Protein-protein Interaction

No.	Protocol	File
011	Immunoprecipitation	100KB
101	Immobilization of proteins on COOH beads	89KB
102	Immobilization of His-Tag proteins on Ts beads	96KB
105	Immobilization of Antibodies or Proteins on NHS beads	80KB
106	Immobilization of Antibodies or Proteins on Epoxy Beads	78KB
107	Direct Quantification of Immobilized Proteins (Antibodies)	102KB
108	Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads	93KB
110	Immobilization of antibodies on Protein A beads and Protein G beads	134KB

Purification of DNA and RNA binding substances

No.	Protocol	File
001	Screening by using ligand immobilized beads	201KB
301	Immobilization of double strand DNA on Plain beads	137KB

Preparation of cell extract

No.	Protocol	File
401	Preparation of cell extract (Large scale method)	148KB
402	Preparation of cell extract (Small scale method)	138KB