Tamagawa seiki Co,.Ltd.

  1. arrow Japanese
  2. arrow English
  3. arrow Site map
  4. arrow Tamagawa seiki web site

Questions about FG beads

  1. Are the sizes of FG beads all equal?

    The sizes are 0.2 μm. The CV value indicating variation is approximately 30%.

  2. What is the linker length of FG beads?

    It is about 1 nm.

  3. What is the number of particles per 1 mg of FG beads?

    It is about 1.8×1011 particles.

  4. What types of the FG beads are there?

    At present, 13 types of FG beads are available, with different surface modificationssuch as functional groups or bound proteins. Additions to the lineup are currently being considered.
    In addition to FG beads, there are also fluorescent beads.

  5. What is the approximate quantity of the functional groups on FG beads?

    The amount of epoxy on Plain beads is 1 μmol per 1 mg of beads. The amount of functional groups on COOH beads and NHS beads is 200 – 300 nmol per 1 mg of beads.

  6. Are the FG beads resistant to heat?

    There are no problems up to approximately 70°C. However the type of beads having high functional reactivity of the linker end may come under an influence by the heat.

  7. Are the FG beads resistant to pH?

    There are no problems within the range of pH2 - pH11. However the protein immobilized on beads may denature and the performance may decrease.

  8. Is there the resistance to organic solvents of FG beads in addition to DMF?

    Ligands can be immobilized on the surfaces of FG beads in various organic solvents, such as methanol, DMF, DMSO, THF, ethyl acetate, pyridine, dioxane, toluene, dichloromethane, chloroform, etc.

  9. Can the FG beads be reused?

    It is believed that the beads can be reused when salt elution or drug elution was performed. However boil elution causes degeneration of the FG beads and makes it impossible for them to be reused.

  10. How should one select a bead type?

    Select the FG beads with the optimal function group according to the substance or protein that you will bind, or select streptavidin beads