The sizes are 0.2 μm. The CV value indicating variation is approximately 30%.
It is about 1 nm.
It is about 1.8×1011 particles.
At present, 13 types of FG beads are available, with different surface
modificationssuch as functional groups or bound proteins. Additions to
the lineup are currently being considered.
In addition to FG beads, there are also fluorescent beads.
The amount of epoxy on Plain beads is 1 μmol per 1 mg of beads. The amount of functional groups on COOH beads and NHS beads is 200 – 300 nmol per 1 mg of beads.
There are no problems up to approximately 70°C. However the type of beads
having high functional reactivity of the linker end may come under an influence
by the heat.
There are no problems within the range of pH2 - pH11. However the protein immobilized on beads may denature and the performance may decrease.
Ligands can be immobilized on the surfaces of FG beads in various organic solvents, such as methanol, DMF, DMSO, THF, ethyl acetate, pyridine, dioxane, toluene, dichloromethane, chloroform, etc.
It is believed that the beads can be reused when salt elution or drug elution was performed. However boil elution causes degeneration of the FG beads and makes it impossible for them to be reused.
Select the FG beads with the optimal function group according to the substance or protein that you will bind, or select streptavidin beads