FF beads/ FS beads

Fluorescent beads

FF beads/ FS beads

FG beads containing fluorescent dye.
There are two types, “FF beads” that have a magnetic material that can be separated magnetically, and “FS beads” that do not have a magnetic material.
There are three types of fluorescent dyes: Europium and 2 types of Cyanine compounds.

FF beads/ FS beads | 蛍光ビーズ

Comparison of FF beads and FS beads

Comparison of FF beads and FS beads Comparison of FF beads and FS beads

 

About fluorescent dyes

In the case of Europium

Europium is characterized by a long-life fluorescent dye life and a very long Stokes shift.
In addition, since the fluorescent dye emits very strong light, it can be expected to be used for high sensitivity of in vitro diagnostic agents.

 

Europium (Excitation(Ex) 340 nm、 Emission(Em) 616 nm)

Europium (Excitation(Ex) 340 nm、 Emission(Em) 616 nm) Europium (Excitation(Ex) 340 nm、 Emission(Em) 616 nm)

 

In the case of Cyanine

Cyanine is a fluorescent dye having fluorescence / excitation wavelength that is widely used in the biotechnology field. It can be used for fluorescent labeling of proteins and FACS.

 

Cyanine3 (Excitation(Ex) 550 nm、Emission(Em) 576 nm)

Cyanine3 (Excitation(Ex) 550 nm、Emission(Em) 576 nm) Cyanine3 (Excitation(Ex) 550 nm、Emission(Em) 576 nm)

 

Cyanine5 (Excitation(Ex) 650 nm、Emission(Em) 684 nm)

Cyanine5 (Excitation(Ex) 650 nm、Emission(Em) 684 nm) Cyanine5 (Excitation(Ex) 650 nm、Emission(Em) 684 nm)

 

Fluorescent beads lineup

※Please scroll horizontally.

Fluorescent dye Functional group FF beads
Magnetic type・180±30 nm
(Code)
FS beads
Non-magnetic type・400±20 nm
(Code)
Price
Europium Carboxylic acid FF Eu COOH beads
(TAB8849N2140)
FS Eu COOH beads
(TAB5849N2140)
Please contact us
Streptavidin FF Eu Streptavidin beads
(TAB8849N2170)
FS Eu Streptavidin beads
(TAB5849N2170)
Protein G FF Eu Protein G beads
(TAB8849N2173)
FS Eu Protein G beads
(TAB5849N2173)
Cyanine 3 Carboxylic acid FF Cyanine3 COOH beads
(TAB8850N2140)
FS Cyanine3 COOH beads
(TAB5850N2140)
Streptavidin FF Cyanine3 Streptavidin beads
(TAB8850N2170)
FS Cyanine3 Streptavidin beads
(TAB5850N2170)
Cyanine 5 Carboxylic acid FF Cyanine5 COOH beads
(TAB8851N2140)
FS Cyanine5 COOH beads
(TAB5851N2140)
Streptavidin FF Cyanine5 Streptavidin beads
(TAB8851N2170)
FS Cyanine5 Streptavidin beads
(TAB5851N2170)

 

Application example

01. Sandwich immunoassay using FF Eu beads

This is an example of application to immunoassay by antibody immobilized FF beads. Since the FF beads contain plenty of fluorescent dye, the detection sensitivity of them has become higher than the labelled antibodies of the conventional immunoassay. In addition, the reaction efficiency and the measuring speed have been improved by using a magnet for antigen-antibody reaction.

Sandwich immunoassay using FF Eu beads

 

Detection result of the α-fetoprotein (tumor marker) in human serum

Detection result of the α-fetoprotein (tumor marker) in human serum

02. Immunostaining using FF Eu beads

When staining of EGFR receptors in a xenograft specimen of A 431 human epidermoid carcinoma cell was performed by using FF Eu beads, the similar result to the result of the conventional immunostaining was obtained in a short time.

Conventional immunostaining

Conventional immunostaining

Immunostaining using FF beads

Immunohistochemical with FF beads

Sakamoto S, et al.Clinical Chemistry, 2014,60:4, 610–620,

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