Protein Identification
- I want to analyze bound proteins with MS, but what should I do if the target protein band is thin?
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Increase the scale of affinity purification (ex: 2.5 mg / 1000 μL), or increase the number of samples under the same conditions to perform affinity purification.
- How much protein can be analyzed by MS?
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It depends on the type of equipment used, but it can be analyzed if the amount of protein is about 50 ng.
