FF beads/ FS beads

Fluorescent beads

FG beads containing fluorescent dye.
There are two types, “FF beads” that have a magnetic material that can be separated magnetically, and “FS beads” that do not have a magnetic material.
There are three types of fluorescent dyes: Europium and 2 types of Cyanine compounds.

Chemical Biology Immunoprecipitation Analysis of Protein-protein Interactions Cell separation Virus / Exosome Isolation Compounds Immobilization Antibodies Immobilization Proteins Immobilization COOH beads Streptavidin beads Protein G beads Contracted services Buffer Magnet stand

Comparison of FF beads and FS beads

About fluorescent dyes

In the case of Europium

Europium is characterized by a long-life fluorescent dye life and a very long Stokes shift.
In addition, since the fluorescent dye emits very strong light, it can be expected to be used for high sensitivity of in vitro diagnostic agents.

Europium (Excitation(Ex) 340 nm、 Emission(Em) 616 nm)

In the case of Cyanine

Cyanine is a fluorescent dye having fluorescence / excitation wavelength that is widely used in the biotechnology field. It can be used for fluorescent labeling of proteins and FACS.

Cyanine3 (Excitation(Ex) 550 nm、Emission(Em) 576 nm)

Cyanine5 (Excitation(Ex) 650 nm、Emission(Em) 684 nm)

Fluorescent beads lineup

※Please scroll horizontally.

Fluorescent dye	Functional group	FF beads Magnetic type・180±30 nm （Code）	FS beads Non-magnetic type・400±20 nm （Code）	Price
Europium	Carboxylic acid	FF Eu COOH beads (TAB8849N2140)	FS Eu COOH beads (TAB5849N2140)	Please contact us
	Streptavidin	FF Eu Streptavidin beads (TAB8849N2170)	FS Eu Streptavidin beads (TAB5849N2170)
	Protein G	FF Eu Protein G beads (TAB8849N2173)	FS Eu Protein G beads (TAB5849N2173)
Cyanine 3	Carboxylic acid	FF Cyanine3 COOH beads (TAB8850N2140)	FS Cyanine3 COOH beads (TAB5850N2140)
Cyanine 3	Streptavidin	FF Cyanine3 Streptavidin beads (TAB8850N2170)	FS Cyanine3 Streptavidin beads (TAB5850N2170)
Cyanine 5	Carboxylic acid	FF Cyanine5 COOH beads (TAB8851N2140)	FS Cyanine5 COOH beads (TAB5851N2140)
Cyanine 5	Streptavidin	FF Cyanine5 Streptavidin beads (TAB8851N2170)	FS Cyanine5 Streptavidin beads (TAB5851N2170)

Application example

01. Sandwich immunoassay using FF Eu beads

This is an example of application to immunoassay by antibody immobilized FF beads. Since the FF beads contain plenty of fluorescent dye, the detection sensitivity of them has become higher than the labelled antibodies of the conventional immunoassay. In addition, the reaction efficiency and the measuring speed have been improved by using a magnet for antigen-antibody reaction.

Sandwich immunoassay using FF Eu beads

Detection result of the α-fetoprotein (tumor marker) in human serum

02. Immunostaining using FF Eu beads

When staining of EGFR receptors in a xenograft specimen of A 431 human epidermoid carcinoma cell was performed by using FF Eu beads, the similar result to the result of the conventional immunostaining was obtained in a short time.