FG beadsは以下の用途でも使用されています。
タンパク質間の相互作用を特定することは、機能未知のタンパク質の働きを知る上で非常に重要です。タンパク質固定化ビーズを用いて回収した標的タンパク質を解析することにより、固定化したタンパク質の機能を明らかにすることができます。
Intelectin1 ameliorates macrophage activation via inhibiting the nuclear factor kappa B pathway
Endocrine Journal 69 (2022) 5 Pages 539-546
Chemical Synthesis of Atomically Tailored SUMO E2 Conjugating Enzymes for the Formation of Covalently Linked SUMO–E2–E3 Ligase Ternary Complexes.
J. Am. Chem. Soc., 141, 37, 14742(2019)
Affinity Chromatographic Materials.
Encyclopedia of Polymeric Nanomaterials, DOI:10.1007 (2014).
Protein fishing using magnetic nano-beads containing calmodulin site-specifically immobilized via an azido-group
J. Biochem., DOI:10.1093 (2013).
Disruption of TBP-2 ameliorates insulin sensitivity and secretion without affecting obesity
Nat. Commun., 1:127 (2010).
The enteropathogenic E. coli effector EspB facilitates microvillus effeacing and anitiphagocytosis by inhibiting myosin function
Cell Host & Microbe, 2, 383 (2007).
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
Nucleic Acids Res., 11, 1475 (1983).
FG beadsは、細胞表面の抗原を認識する抗体を使用することで大きな装置を必要とせず、磁気回収により簡単に短時間で細胞を回収することが可能です。FG beadsへ抗体を固定化した後に細胞へ添加する直接法や、細胞を先に抗体で標識した後にビーズを添加する間接法のどちらも選択可能です。
Age-related dysfunction of p53-regulated phagocytic activity in macrophages
Biochemical and Biophysical Research Communications 529 (2020) 462
DNAやRNAを固定化したビーズを用いて、次のようなことができます。
その他の方法として、DNAやRNAの末端へ官能基を導入して固定化する方法があります。
インタビュー ‐東京医科大学 半田宏先生
詳しく見る→
Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding RNAs
Epigenetics & Chromatin (2021) 14:46
Itpr1 regulates the formation of anterior eye segment tissues derived from neural crest cells
Development (2021) 148 (16): dev188755
Comparative analysis of type 2 diabetes-associated SNP alleles identifies allele-specific DNA-binding proteins for the KCNQ1 locus
Int J Mol Med. 2015 Jul;36(1):222-30.
DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells
Chemistry & Biology 22 (2015) 1671–1679
Affinity Chromatographic Materials.
Encyclopedia of Polymeric Nanomaterials, DOI:10.1007 (2014).
M. Golan-Mashiach et al. (2012)
Identification of CTCF as a master regulator of the clustered protocadherin genes
Nucl. Acids Res., 40, 3378 (2012).
A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress
Genes Dev., 23, 208 (2009).
Regulation of Immunoglobulin Promoter Activity by TFII-I Class Transcription Factors
J. Biol. Chem., 279, 5460 (2004).
Embryonic Lethality, Decreased Erythropoiesis, and Defective Octamer-Dependent Promoter Activation in Oct-1-Deficient Mice
Mol. Cell. Biol., 24, 1022 (2004).
Total Analysis and Purification of Cellular Proteins Binding to Cisplatin-Damaged DNA Using Submicron Beads
Bioconjugate Chem., 13, 163 (2002).
Copurification of casein kinase II with transcription factor ATF/E4TF3
Nucleic Acids Res., 24, 876 (1996).
Selective isolation of DNA or RNA using single-stranded DNA affinity latex particles
J. Colloid Interface Sci., 177, 245 (1996).
[40] DNA affinity chromatography
Methods in Enzymology, 254, 595 (1995).
Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex
J. Biomater. Sci. Polym. Ed., 5, 293 (1994).
Direct purification of multiple ATF/E4TF3 polypeptides from HeLa cell crude nuclear extracts using DNA affinity latex particles
Anal. Biochem., 206, 109 (1992).
A Common trans-Acting Factor, Ad4-binding Protein, to the Promoters of Steroidogenic P-450s
J. Biol. Chem., 267, 17913 (1992).
Purification of DNA-binding transcription factors by their selective adsorption on the affinity latex particles
Nucleic Acids Res., 17, 6229 (1989).
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
Nucleic Acids Res., 11, 1475 (1983).
ビーズへターゲットの表面抗原を認識する抗体を固定化し、ウイルスやエクソソームを分離します。
Quick and ultra-sensitive digital assay of influenza virus using sub-picoliter microwells
Analytica Chimica Acta Volume 1213, 22 June 2022, 339926
Y. Kabe et al. (2019) [ExoCounter]
Application of high-performance magnetic nanobeads to biological sensing devices.
Aal. Bioanal. Chem., doi: 10.1007/s00216-018-1548-y (2019).
Y. Kabe et al. (2018) [ExoCounter]
Development of a Highly Sensitive Device for Counting the Number of Disease-Specific Exosomes in Human Sera
Clinical Chemistry, DOI: 10.1373/clinchem.2018.291963 (2018).
SR Kristensen et al. (2018) [ExoCounter]
Blu-ray beyond music and movies—novel approach to diagnostics measuring specific extracellular vesicles
Journal of Laboratory and Precision Medicine 84 (2018) 3
Development of an efficient entire-capsid-coding-region amplification method for direct detection of poliovirus from stool extracts
J. Clin. Microbiol., 53, 73 (2015).
Tsujita et al.(2013) [ExoCounter]
Ultrahigh-Sensitivity Biomarker Sensing System Based on the Combination of Optical Disc Technologies and Nanobead Technologies
Japanese Journal of Applied Physics 52 (2013) 09LB02
Development of poliovirus extraction method from the stool extracts by using magnetic nanoparticles sensitized with soluble poliovirus receptor.
J. Clin. Microbiol. DOI:10.1128 (2013).
ビーズへ標的タンパク質を固定化し、その標的タンパク質に特異的な相互作用のあるペプチド（またはタンパク質）を持つファージを利用して精製します。
インタビュー ‐電気通信大学 瀧真澄先生、北陸先端科学技術大学院大学 福永圭佑先生
詳しく見る→
Directed evolution of orthogonal RNA–RBP pairs through library-vs-library in vitro selection
Nucleic Acids Research, gkab527 (2021)
Combinatorially Screened Peptide as Targeted Covalent Binder:Alteration of Bait-Conjugated Peptide to Reactive Modifier
Bioconjugate Chem., 29, 1866 (2018)
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