Protocols

Chemical Biology

001

Screening by using ligand immobilized beads

003

Immobilization of ligands (compounds with phenol groups or NH₂ groups) on epoxy beads

003S

Immobilization of ligands (compounds with phenol groups or NH₂ groups) on epoxy beads (Small scale method)

004

Immobilization of ligands (carboxylic compounds) on OH beads

004S

Immobilization of ligands (carboxylic compounds) on OH beads (Small scale method)

005

Immobilization of ligands (carboxylic compounds) on NH₂ beads

005S

Immobilization of ligands (carboxylic compounds) on NH₂ beads (Small scale method)

008

Immobilization of ligands (compounds with NH₂ groups) on COOH beads

008S

Immobilization of ligands (compounds with NH₂ groups) on COOH beads (Small scale method)

012

Competitive inhibition

013

Drug elution

014

Immobilization of ligands (compounds with NH₂ groups) on NHS beads

014S

Immobilization of ligands (compounds with NH₂ groups) on NHS beads (Small scale method)

016

Immobilization of ligands (compounds with OH groups) on COOH beads

108

Immobilization of biotinylated molecule on Streptavidin beads and NeutrAvidin beads

109

Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction

109S

Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction (Small scale method)

111

Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction

111S

Immobilization of ligands (azide structure compounds) on alkyne beads using click chemistry reaction (Small scale method)

201

Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography)

401

Preparation of cell extract (Large scale method)

402

Preparation of cell extract (Small scale method)