{"id":18,"date":"2022-05-09T13:57:37","date_gmt":"2022-05-09T04:57:37","guid":{"rendered":"https:\/\/www.fgb.tamagawa-seiki.com\/english\/?page_id=18"},"modified":"2022-06-16T11:12:04","modified_gmt":"2022-06-16T02:12:04","slug":"immunoprecipitation","status":"publish","type":"page","link":"https:\/\/fgb.tamagawa-seiki.com\/english\/selection\/immunoprecipitation","title":{"rendered":"Immunoprecipitation"},"content":{"rendered":"
\n
\n

By using FG beads and utilizing the immunoprecipitation reaction by specific binding between antigen and antibody, it can be applied to a wide range of fields such as detection, separation and purification of antigen and antibody.<\/p>\n

Select beads from Immobilization Target or Immobilization Method<\/h2>\n

Select from ligands to be immobilized<\/h3>\n

\uff081\uff09Antibody<\/h4>\n

The antibodies are immobilized on the beads by affinity or covalent bond.<\/p>\n

\"\uff081\uff09Antibody\"<\/p>\n

\n
List of corresponding products<\/h5>\n
\n

\u203bPlease scroll horizontally.<\/p>\n\n\n\n\n\n
<\/th>\nImmobilization to NH2<\/sub> group of protein<\/th>\nImmobilization with affinity binding<\/th>\nImmobilized with Avidin-biotin binding<\/th>\nImmobilization to SH group and NH2<\/sub> group of protein<\/th>\nImmobilization to His-tag of protein<\/th>\nImmobilization with click chemistry<\/th>\n<\/tr>\n
It is possible to firmly immobilize the proteins by covalent bond to the beads. Since the NH2<\/sub> groups on the protein is randomly immobilized on the beads, the orientation of the antibodies becomes disjointed. NHS beads are recommended because they are easier to operate than COOH beads.<\/td>\nOnly antibodies can be immobilized on beads. The Fc site of the antibody binds to the beads by affinity. The immobilization operation is easy.<\/td>\nImmobilizes to beads with strong affinity binding. Immobilization requires biotinlation of the ligand.<\/td>\nLigand immobilization is possible with inexpensive beads. However, temperature tolerance of the protein at 37\u2103 is required.<\/td>\nIt is necessary to introduce His-tag into the protein. The beads also bind to the NH2<\/sub> groups on the protein.<\/td>\nIt is necessary to introduce His-tag into the protein. The beads also bind to the NH2<\/sub> groups on the protein.<\/td>\n<\/tr>\n
\uff081\uff09Antibody<\/td>\n\u25ce<\/td>\n\u25ce<\/td>\n\u25ce<\/td>\n\u25cb<\/td>\n\u25cb<\/td>\n\u25b3<\/td>\n<\/tr>\n
Recommended beads<\/td>\nCOOH beads<\/a>NHS beads<\/a><\/td>\nProtein A beads<\/a>Protein G beads<\/a><\/td>\nStreptavidin beads<\/a>NeutrAvidin beads<\/a><\/td>\nEpoxy beads<\/a><\/td>\nTs beads<\/a><\/td>\nAzide beads<\/a>Alkyne beads<\/a><\/td>\n<\/tr>\n<\/table>\n

\u25ce\uff1aRecommended for use\u3000\u3007\uff1aAvailable\u3000\u25b3\uff1aCan be used depending on the conditions\u3000\u00d7\uff1aNot available<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<\/section>\n

\n
\n

\uff082\uff09Antigen<\/h4>\n

The antigens are immobilized on the beads by affinity or covalent bond.<\/p>\n

\"\uff082\uff09Antigen\"<\/p>\n

\n
List of corresponding products<\/h5>\n
\n

\u203bPlease scroll horizontally.<\/p>\n\n\n\n\n\n
<\/th>\nImmobilization to NH2<\/sub> group of protein<\/th>\nImmobilization with affinity binding<\/th>\nImmobilized with Avidin-biotin binding<\/th>\nImmobilization to SH group and NH2<\/sub> group of protein<\/th>\nImmobilization to His-tag of protein<\/th>\nImmobilization with click chemistry<\/th>\n<\/tr>\n
It is possible to firmly immobilize the proteins by covalent bond to the beads. Since the NH2<\/sub> groups on the protein is randomly immobilized on the beads, the orientation of the antibodies becomes disjointed. NHS beads are recommended because they are easier to operate than COOH beads.<\/td>\nOnly antibodies can be immobilized on beads. The Fc site of the antibody binds to the beads by affinity. The immobilization operation is easy.<\/td>\nImmobilizes to beads with strong affinity binding. Immobilization requires biotinlation of the ligand.<\/td>\nLigand immobilization is possible with inexpensive beads. However, temperature tolerance of the protein at 37\u2103 is required.<\/td>\nIt is necessary to introduce His-tag into the protein. The beads also bind to the NH2<\/sub> groups on the protein.<\/td>\nIt is necessary to introduce His-tag into the protein. The beads also bind to the NH2<\/sub> groups on the protein.<\/td>\n<\/tr>\n
\uff082\uff09Antigen<\/td>\n\u25ce<\/td>\n\u00d7<\/td>\n\u25ce<\/td>\n\u25cb<\/td>\n\u25cb<\/td>\n\u25b3<\/td>\n<\/tr>\n
Recommended beads<\/td>\nCOOH beads<\/a>NHS beads<\/a><\/td>\nProtein A beads<\/a>Protein G beads<\/a><\/td>\nStreptavidin beads<\/a>NeutrAvidin beads<\/a><\/td>\nEpoxy beads<\/a><\/td>\nTs beads<\/a><\/td>\nAzide beads<\/a>Alkyne beads<\/a><\/td>\n<\/tr>\n<\/table>\n

\u25ce\uff1aRecommended for use\u3000\u3007\uff1aAvailable\u3000\u25b3\uff1aCan be used depending on the conditions\u3000\u00d7\uff1aNot available<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<\/div>\n<\/section>\n

\n
\n

Selected from immobilization or purification methods<\/h3>\n

\uff083\uff09Direct method<\/h4>\n

After immobilizing the antibodies (or antigens) on the beads, the target antigens (or antibodies) are recovered (bound).<\/p>\n

\"\uff083\uff09Direct<\/p>\n