{"id":10,"date":"2022-05-09T13:55:45","date_gmt":"2022-05-09T04:55:45","guid":{"rendered":"https:\/\/www.fgb.tamagawa-seiki.com\/english\/?page_id=10"},"modified":"2022-06-15T18:17:35","modified_gmt":"2022-06-15T09:17:35","slug":"case_study","status":"publish","type":"page","link":"https:\/\/fgb.tamagawa-seiki.com\/english\/case_study","title":{"rendered":"Identification Examples of Target Protein by FG beads"},"content":{"rendered":"
\n
\n

Introduction of identification examples using FG beads<\/span>.<\/h2>\n<\/div>\n<\/section>\n
\n
\n

Example 01<\/span>Search for Novel Target Proteins of Thalidomide<\/span><\/h2>\n
\n

When thalidomide was immobilized on FG beads and affinity purification was performed from human cell extract, cereblon (CRBN) and DDB1 were identified. Cereblon is a constituent of an enzyme involved in protein degradation, and thalidomide has been found to cause teratogenicity by inhibiting the action of this enzyme.<\/p>\n

Reference:T. Ito et al., Science 327 (2010) 1345<\/a><\/p>\n<\/p><\/div>\n

\"Search\n<\/div>\n<\/section>\n

\n
\n

Example 02<\/span>Search for Target Protein of Capsaicin<\/span><\/h2>\n
\n

When a capsaicin derivative (Cap-NH2<\/sub>) was immobilized on FG beads and affinity purification was performed from human leukemia NB4 cells, Prohibitin1 and Prohibitin2 were identified. From this result the mechanism was elucidated by which capsaicin induces apoptosis in leukemia cells.<\/p>\n

Reference:C. Kuramori et al., Biochem. Biophys. Res. Commun. 379 (2009) 519<\/a><\/p>\n<\/p><\/div>\n

\"Search\n<\/div>\n<\/section>\n

\n
\n

Example 03<\/span>Search for Novel Target Proteins for MTX (methotrexate)<\/span><\/h2>\n
\n

The immobilization site of MTX was changed and immobilized on FG beads. Each target protein was subject to affinity purification from cancer cell extract. The target proteins DHFR (dihydrofolate reductase) and dCK (deoxycytidine kinase) were purified by different immobilization sites. From this result the mechanism of anticancer action of MTX was elucidated in detail.<\/p>\n

Reference:H. Uga et al., Mol. Pharmacol. 70 (2006) 1832<\/a><\/p>\n

\"Search\n <\/div>\n

\"Search\n<\/div>\n<\/section>\n

\n
\n
\n

Example 04<\/span>Search for Target Protein of EspB Protein<\/span><\/h2>\n
\n

The EspB protein of pathogenic Escherichia coli was immobilized on the beads, and the affinity was purified from the human cell extract. It could be confirmed that the EspB protein to bind to Myosin1C and inhibit its function.<\/p>\n

Reference:Y. Iizumi et al., Cell Host & Microbe. 2 (2007) 383<\/a><\/p>\n<\/p><\/div>\n

\t\t\"ThalidomideSearch\n\t<\/div>\n

For other examples, see<\/p>\n

Papers\/Technical Information<\/span><\/a><\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"

Introduction of identification examples using FG beads. Example 01Search for Novel Target Proteins of Thalidom […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/pages\/10"}],"collection":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/comments?post=10"}],"version-history":[{"count":15,"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/pages\/10\/revisions"}],"predecessor-version":[{"id":1040,"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/pages\/10\/revisions\/1040"}],"wp:attachment":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/media?parent=10"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}