{"id":228,"date":"2022-05-16T12:53:50","date_gmt":"2022-05-16T03:53:50","guid":{"rendered":"https:\/\/www.fgb.tamagawa-seiki.com\/english\/?post_type=faq&#038;p=228"},"modified":"2022-06-16T15:04:58","modified_gmt":"2022-06-16T06:04:58","slug":"how-is-the-cell-extract-prepared","status":"publish","type":"faq","link":"https:\/\/fgb.tamagawa-seiki.com\/english\/faq\/how-is-the-cell-extract-prepared","title":{"rendered":"How is the cell extract prepared?"},"content":{"rendered":"<p>We recommend the Dignam method. However, since the Dignam method requires a large amount of cells, we recommend a method of solubilizing with a surfactant such as NP-40 when using a small amount. Please refer to the <a href=\"https:\/\/fgb.tamagawa-seiki.com\/english\/protocol\/preparation-of-cell-extract-large-scale-method\">protocols 401<\/a> and <a href=\"https:\/\/fgb.tamagawa-seiki.com\/english\/protocol\/preparation-of-cell-extract-small-scale-method\">402<\/a> from the <a href=\"https:\/\/fgb.tamagawa-seiki.com\/english\/protocol\/\">protocol page<\/a>. If the above is difficult, you can also use commercially available kits (ProteoExtract Subcellular Proteome Extraction Kit (MERCK MILLIPORE), CelLytic M (SIGMA), etc.). However, if the detergent concentration is 1% or higher, it may prevent the ligand from binding to the target protein during affinity purification, so reduce the detergent concentration to 0.1% by dialysis or dilution before use.<\/p>\n","protected":false},"featured_media":0,"parent":0,"template":"","acf":[],"_links":{"self":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/faq\/228"}],"collection":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/faq"}],"about":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/types\/faq"}],"wp:attachment":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/media?parent=228"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}