{"id":212,"date":"2022-05-16T12:12:21","date_gmt":"2022-05-16T03:12:21","guid":{"rendered":"https:\/\/www.fgb.tamagawa-seiki.com\/english\/?post_type=faq&#038;p=212"},"modified":"2022-08-10T14:32:59","modified_gmt":"2022-08-10T05:32:59","slug":"is-there-a-way-to-increase-the-antibody-immobilization-efficiency-immobilization-amount","status":"publish","type":"faq","link":"https:\/\/fgb.tamagawa-seiki.com\/english\/faq\/is-there-a-way-to-increase-the-antibody-immobilization-efficiency-immobilization-amount","title":{"rendered":"Is there a way to increase the antibody immobilization efficiency (immobilization amount)?"},"content":{"rendered":"<p>There are several ways. Please refer to the following.<\/p>\n<p>(1) In the case of NHS beads, the amount of immobilization may increase by lengthening the immobilization reaction time. However, the NHS group is susceptible to hydrolysis and causes non-specific adsorption, so the reaction time should be within 6 hours.<\/p>\n<p>(2) In the case of NHS beads, the immobilization reaction may be hindered by a composition other than the recommended immobilization buffer. If the antibody storage solution contains additives such as salts or glycerol, buffer substitution will improve the immobilization efficiency.<\/p>\n<p>(3) The amount of immobilization increases by increasing the amount of antibody added during the immobilization reaction.<\/p>\n","protected":false},"featured_media":0,"parent":0,"template":"","acf":[],"_links":{"self":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/faq\/212"}],"collection":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/faq"}],"about":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/types\/faq"}],"wp:attachment":[{"href":"https:\/\/fgb.tamagawa-seiki.com\/english\/wp-json\/wp\/v2\/media?parent=212"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}