CRBN (Cereblon) and DDB1 are isolated from human cell extract by using thalidomide-immobilized beads. As a result, the teratogenic mechanism of thalidomide was elucidated.
Prohibitin1 and Prohibitin2 are isolated from human myeloid leukemia NB4 cell extract by using capsaicin derivative (Cap-NH2) fixed beads. As a result, the apoptosis induction mechanism of capsaicin was elucidated.
When MTX is fixed via different site, a novel protein is purified and identified as deoxycytidine kinase (dCK). As a result, a possible mechanism of synergistic effect between MTX and ara-C on malignant lymphoma was proposed.
EspB is a protein of entropathogenic E. coli(EPEC) essential for infection in humans. Myosin is isolated from human cell extract by using EspB-immobilized beads. As a result, the mechanism of EPEC infection was elucidated.