FG beads® are materices for affinity purification that provide superior characteristics which are not available with conventional materices. These characteristics include the following.
The nano size of FG beads® gives them excellent dispersibility. The ligands on the surface of the beads efficiently bind to the target protein suspended in solution, yielding a higher recovery rate.
Because of their nano size, FG beads® have a large surface area per unit of volume. This allows many ligands to be immobilized on them, allowing collection of large amounts of the target protein.
The surface of the FG beads® is coated with a special hydrophilic polymer called poly-GMA (glycidyl methacrylate), which results in extremely little non-specific protein adsorption, and allows the target protein to be purified with a high degree of purity.
FG beads® are resistant to various types of organic solvents, and the beads do not degenerate or dissolve in them. A variety of ligands can be immobilized on the beads in an organic solvent. The organic solvents which can be used include methanol, DMF, DMSO, THF, ethyl acetate, pyridine, dioxane, toluene, dichloromethane, and chloroform.
Because the FG beads® contain magnetic ferrite, they can be recovered by magnet. Recovery by magnet allows the target proteins to be recovered without effects from factors such as large proteins and degenerated proteins. The use of an automated screening system allows the affinity purification process to be automated, and allows multiple samples to be processed simultaneously, shortening the screening time.